Composite

Part:BBa_K5055004:Design

Designed by: Laurine Wachspress   Group: iGEM24_Ionis-Paris   (2024-09-30)


Tobacco Mosaic Virus coat protein composite part


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal BamHI site found at 642
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 581


Design Notes

To choose our design, we selected the T7 promoter and terminator because we planned to produce our protein with the E. coli strain BL21. This strain can produce the T7 polymerase when cultured with IPTG, which is specific for its own promoter and terminator.


Source

The coding sequence from this part comes from the Tobacco Mosaic Sequence, the sequence was found on NCBI (coat protein [ Tobacco mosaic virus ]).

References